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            浮游植物分類熒光儀Phyto-PAM
            浮游植物分類熒光儀Phyto-PAM

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            PHYTO‑PAM 全球**款可自動(dòng)對(duì)浮游植物分類的熒光儀

            有害藻華(HABs)監(jiān)測(cè)/預(yù)警的強(qiáng)大工具

            主要功能

            1)對(duì)自然水體中的藍(lán)藻、綠藻和硅/甲藻自動(dòng)分類(定性)

            2)自動(dòng)測(cè)量水樣中藍(lán)藻、綠藻和硅/甲藻的葉綠素a含量(定量)和總?cè)~綠素a含量

            3)一杯自然水樣,同時(shí)獲得藍(lán)藻、綠藻和硅/甲藻的光合活性:

            * 光合效率和光合速率(相對(duì)電子傳遞速率)

            * 快速光曲線并進(jìn)行擬合

            * 藻類的潛在**光合效率(“生長(zhǎng)潛能”)

            * 藻類的光保護(hù)能力

            * 藻類耐受強(qiáng)光的能力

            4)用戶可做自己的參考光譜

            應(yīng)用領(lǐng)域

            主要用于水生生物學(xué)、水域生態(tài)學(xué)、海洋學(xué)、湖沼學(xué)、水質(zhì)預(yù)警、微藻生理學(xué)、微藻抗逆性等領(lǐng)域,對(duì)于了解自然水體中藻類種群的動(dòng)態(tài)變化、水華預(yù)警、野外水體中光合作用的時(shí)空變化、校正初級(jí)生產(chǎn)力的計(jì)算等有較大幫助。

            特別適于浮游植物動(dòng)力學(xué)研究和有害藻華(HABs)的早期預(yù)警。

            測(cè)量參數(shù)

            Fo, Fm, F, Fm', Fv/Fm, Y(II)=ΔF/Fm', ETR, a, Ik, Pm, PAR、藍(lán)藻Chla含量、綠藻Chla含量、硅/甲藻Chla含量、總Chla含量等

            特點(diǎn)

            1) 全世界**臺(tái)可對(duì)浮游植物自動(dòng)分類的調(diào)制葉綠素?zé)晒鈨x

            2) 4波長(zhǎng)光源:470、520、645和665 nm

            3) 對(duì)藍(lán)藻、綠藻和硅/甲藻進(jìn)行分類

            4) 可選配室內(nèi)系統(tǒng)(I)、野外系統(tǒng)(II)和測(cè)附著藻類/大型藻類的系統(tǒng)(III)

            5) 靈敏度高,檢測(cè)限為0.1 μg L-1 Chl

            6) 專業(yè)PhytoWin操作軟件,數(shù)據(jù)收集、分析和存貯功能強(qiáng)大

            7) 用戶可利用培養(yǎng)的微藻做參考光譜,非“黑匣子”

            8) 可在野外測(cè)量后根據(jù)水體藻類組成利用優(yōu)勢(shì)種(一種或多種)的參考光譜校對(duì)實(shí)驗(yàn)結(jié)果

            利用PHYTO-PAM進(jìn)行水華預(yù)警的原理

            藻類的生長(zhǎng)靠光合作用,藻華的爆發(fā)是在特定的環(huán)境條件下(富營(yíng)養(yǎng)、高光、高溫)由藻類短期快速暴增造成的,這其間藻類必須具備極強(qiáng)的光合作用才能快速生長(zhǎng)。監(jiān)測(cè)葉綠素a含量可以了解目前水體中的藻類生物量,但這只代表歷史(如果營(yíng)養(yǎng)鹽很低,即使當(dāng)前藻類生物量高,也不具備發(fā)生藻華的可能);而監(jiān)測(cè)藻類的光合作用活性可以了解藻類的“生長(zhǎng)潛能”,結(jié)合其它環(huán)境條件可以預(yù)測(cè)未來(富營(yíng)養(yǎng)條件且高光高溫下,即使當(dāng)前藻類生物量不高,但只要光合作用活性強(qiáng),就具有極大的發(fā)生藻華的可能)。

            由于PHYTO-PAM可以測(cè)量自然水樣中藍(lán)藻、綠藻和硅/甲藻各自的光合作用,就可以對(duì)藻華發(fā)生時(shí)不同藻類類群進(jìn)行分析。利用PHYTO-PAM測(cè)量不同藻類葉綠素a含量和光合作用活性的功能,可以長(zhǎng)期監(jiān)測(cè)自然水體中浮游植物種群生物量的動(dòng)力學(xué)變化不同類群光合作用潛力的變化趨勢(shì),這對(duì)于藻華的預(yù)警具有重要參考價(jià)值。

            推薦閱讀:有害藻華(HABs)監(jiān)測(cè)/預(yù)警的新解決方案

            PHYTO-PAM*常用的光合作用參數(shù)

            Fv/Fm,浮游植物的潛在**光合效率(“生長(zhǎng)潛能”)

            Y,給定光強(qiáng)下浮游植物的實(shí)際光合效率

            NPQ,浮游植物將過剩光能耗散為熱的能力,即光保護(hù)能力

            ETR,給定光強(qiáng)下浮游植物的實(shí)際光合速率

            ETRmax,浮游植物的潛在**光合速率

            a,浮游植物對(duì)光強(qiáng)的利用能力

            Ik,浮游植物耐受強(qiáng)光的能力

            快速光曲線,結(jié)合水體光場(chǎng)可用于計(jì)算水體初級(jí)生產(chǎn)力

            利用PHYTO-PAM對(duì)水體長(zhǎng)期監(jiān)測(cè)的方法

            設(shè)計(jì)為大時(shí)間尺度,采樣頻率為每月一次,頻率越高越好。采樣時(shí)可設(shè)計(jì)多個(gè)樣點(diǎn),每個(gè)樣點(diǎn)都分層采樣測(cè)量。這樣就可測(cè)量藍(lán)藻Chla、綠藻Chla、硅/甲藻Chla、總Chla、Fv/Fm、Ik、NPQ等的時(shí)間和空間動(dòng)態(tài)變化,獲知三大類群的浮游植物生物量、“生長(zhǎng)潛能”、耐受強(qiáng)光的能力、光保護(hù)能力等的時(shí)空動(dòng)態(tài)變化,提前預(yù)判其變化趨勢(shì),結(jié)合其它水質(zhì)氣象指標(biāo),進(jìn)行早期的藻華預(yù)警。

            應(yīng)用實(shí)例一太湖藍(lán)藻水華成因分析

            2007年,太湖發(fā)生了嚴(yán)重的藍(lán)藻水華,在國(guó)內(nèi)外引起廣泛關(guān)注。藍(lán)藻水華爆發(fā)的一個(gè)重要原因是周邊地區(qū)往太湖中排污過多,造成湖泊嚴(yán)重富營(yíng)養(yǎng)化,在適宜的光照和溫度條件下藻類瘋長(zhǎng)形成水華。但是太湖中的藻類不僅僅包括藍(lán)藻,也有綠藻、硅藻、甲藻等,為什么總是爆發(fā)藍(lán)藻水華,其它藻并不形成水華呢?中國(guó)科學(xué)院南京地理與湖泊研究所湖泊與環(huán)境國(guó)家重點(diǎn)實(shí)驗(yàn)室科研人員利用可對(duì)自然水體中的藻類定性、定量并測(cè)量光合作用活性的浮游植物熒光儀PHYTO-PAM,探討了藍(lán)藻在太湖中爆發(fā)水華的原因。主要研究結(jié)果如下:光作為藻類生長(zhǎng)的重要能量來源,浮游藻類光利用效率的不同對(duì)水體中浮游藻類初級(jí)生產(chǎn)力、群落組成以及種群演替具有重要影響。本研究發(fā)現(xiàn)藍(lán)藻、綠藻、硅/甲藻三種具有不同的對(duì)光照和垂直混合的響應(yīng)策略,藍(lán)藻的強(qiáng)光耐受能力以及對(duì)過剩光能的耗散能力均超過其他兩種藻;同時(shí)藍(lán)藻主要聚集在表層到0.3 m的深度,而在此深度藻類具有更高的生長(zhǎng)速率,綠藻和硅/甲藻則由于垂直混合和自身調(diào)節(jié)等作用的作用下,不具備藍(lán)藻這一優(yōu)勢(shì),這可能是富營(yíng)養(yǎng)化水體中藍(lán)藻占據(jù)優(yōu)勢(shì)的原因之一。(Zhang M, Kong FX, Wu X, Xing P. Different photochemical responses of phytoplankters from the large shallow Taihu Lake of subtropical China in relation to light and mixing.Hydrobiologia 2008, 603:267-278.)

            應(yīng)用實(shí)例二微囊藻低溫弱光環(huán)境下過冬機(jī)理

            經(jīng)常發(fā)生水華的微囊藻在冬天會(huì)沉降到底泥中進(jìn)行越冬。底泥屬于低溫弱光環(huán)境,在這么苛刻的環(huán)境下微囊藻是怎么越冬的,目前了解的不多。中國(guó)科學(xué)院水生生物研究所淡水生態(tài)與生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室科研人員利用人工培養(yǎng)的單細(xì)胞銅綠微囊藻、群體銅綠微囊藻和斯尾柵藻進(jìn)行了低溫弱光環(huán)境下的耐受力和復(fù)壯實(shí)驗(yàn),其中光合作用活性的測(cè)量利用浮游植物熒光儀PHYTO-PAM進(jìn)行。結(jié)果發(fā)現(xiàn)經(jīng)過30天的低溫弱光環(huán)境處理后,柵藻的光合活力受到顯著抑制,而微囊藻僅受到輕微影響,且群體微囊藻細(xì)胞比單細(xì)胞微囊藻的耐受力更強(qiáng)。復(fù)壯培養(yǎng)后,柵藻的回復(fù)速度和生長(zhǎng)潛力明顯低于微囊藻。這對(duì)于分析微囊藻的越冬機(jī)理和水華機(jī)理具有重要參考意義。(Wu Z, Song L, Li R. Different tolerances and responses to low temperature and darkness between waterbloom forming cyanobacterium Microcystis and a green alga Scenedesmus Hydrobiologia 2008, 596:47-55.)

            選購(gòu)指南

            ● 基礎(chǔ)配置

            ○ 可選配置

            系統(tǒng)I

            (實(shí)驗(yàn)室版)

            系統(tǒng)II

            (野外版)

            系統(tǒng)III

            (光纖版)

            主機(jī)PHYTO-C
            測(cè)量光LED陣列PHYTO-ML
            光化光LED陣列PHYTO-AL
            光電倍增管PM-101P
            光學(xué)單元ED-101US/MP
            工作臺(tái)ST-101
            激發(fā)-檢測(cè)單元PHYTO-ED
            光纖型激發(fā)-檢測(cè)單元PHYTO-EDF
            微型磁力攪拌器PHYTO-MS
            球狀微型光量子探頭US-SQS
            溫度控制器US-T
            攪拌器WATER-S

            主要技術(shù)參數(shù)

            測(cè)量光:波長(zhǎng)470、520、645和665 nm的測(cè)量光LED。

            光化光:波長(zhǎng)655 nm的LED;光化光強(qiáng)度0~2000 μmol m-2 s-1 PAR(系統(tǒng)I和II)或0~1300 μmol m-2 s-1 PAR(系統(tǒng)III)。

            飽和脈沖:波長(zhǎng)655 nm的LED;飽和脈沖強(qiáng)度4000 μmol m-2 s-1 PAR(系統(tǒng)I和II)或2600 μmol m-2 s-1 PAR(系統(tǒng)III)。

            信號(hào)檢測(cè):光電倍增管,帶短波截止濾光片(λ>710 nm);選擇性鎖相放大器。

            測(cè)量參數(shù):Ft, F(或Fo), Fm(或 Fm’), ΔF, Y(ΔF/ Fm’或Fv/Fm), ETR和Chl濃度等。

            環(huán)境溫度:-5~+45 ℃,已在極地成功應(yīng)用。

            部分文獻(xiàn)

            1.Guasch H, Atli G, Bonet B, Corcoll N, Leira M, Serra A: Discharge and the response of biofilms to metal exposure in Mediterranean rivers. Hydrobiologia2010:in press.[PHYTO-PAM]

            2.Liu Y, Wang W, Zhang M, Xing P, Yang Z: PSII-efficiency, polysaccharide production, and phenotypic plasticity of Scenedesmus obliquus in response to changes in metabolic carbon flux Biochemical Systematics and Ecology2010:in press.[PHYTO-PAM]

            3.Pesce S, Margoum C, Montuelle B: In situ relationships between spatio-temporal variations in diuron concentrations and phototrophic biofilm tolerance in a contaminated river. Water Research2010, 44:1941-1949.[PHYTO-PAM]

            4.Soares MCS, Lürling M, Huszar VLM: Responses of the rotifer Brachionus calyciflorus to two tropical toxic cyanobacteria (Cylindrospermopsis raciborskii and Microcystis aeruginosa) in pure and mixed diets with green algae. Journal of Plankton Research2010:in press.[PHYTO-PAM]

            5.van Ruth PD, Ganf GG, Ward iM: The influence of mixing on primary productivity: A unique application of classical critical depth theory Progress In Oceanography2010:in press.[PHYTO-PAM]

            6.Wang H, Liu L, Liu ZP, Qin S: Investigations of the characteristics and mode of action of an algalytic bacterium isolated from Tai Lake. Journal of Applied Phycology2010:in press.[PHYTO-PAM]

            7.Zhu J, Liu B, Wang J, Gao Y, Wu Z: Study on the mechanism of allelopathic influence on cyanobacteria and chlorophytes by submerged macrophyte (Myriophyllum spicatum) and its secretion Aquatic Toxicology2010:in press.[PHYTO-PAM]

            8.任秋芳, 阿依巧麗, 智朱, 張義方, 波曾: 三峽庫(kù)區(qū)季節(jié)及養(yǎng)分對(duì)銅綠微囊藻生長(zhǎng)的影響——模擬烏江回水區(qū)水環(huán)境的研究. 重慶師范大學(xué)學(xué)報(bào)2010, 27(1):1-4.[PHYTO-PAM]

            9.Aikawa S, Hattori H, Gomi Y, Watanabe K, Kudoh S, Kashino Y, Satoh K: Diel tuning of photosynthetic systems in ice algae at Saroma-ko Lagoon, Hokkaido, Japan Polar Science2009, 3(1):57-72.[PHYTO-PAM]

            10.Dimier C, Brunet C, Geider R, Raven J: Growth and photoregulation dynamics of the picoeukaryote Pelagomonas calceolata in fluctuating light Limnology and Oceanography2009, 59(3):823-836.[PHYTO-PAM]

            11.Franklin D, Choi CJ, Hughes C, Malin G, Berges JA: Effect of dead phytoplankton cells on the apparent efficiency of photosystem II. Marine Ecology Progress Series2009, 382:35-40.[PHYTO-PAM]

            12.Hall SR, Becker CR, Simonis JL, Duffy MA, Tessier AJ, Cáceres CE: Friendly competition: evidence for a dilution effect among competitors in a planktonic host–parasite system. Ecology2009, 90(6):1441-1448.[PHYTO-PAM]

            13.Izagirre O, Serra A, Guasch H, Elosegi A: Effects of sediment deposition on periphytic biomass, photosynthetic activity and algal community structure. Science of The Total Environment2009, 407(21):5694-5700.[PHYTO-PAM]

            14.Lee Y, Kang C, Kwon K, Kim S: Organic and inorganic matter increase related to eutrophication in Gamak Bay, South Korea Journal of Environmental Biology 2009, 30(3):373-380.[PHYTO-PAM]

            15.Lee YS, Kim JD, Lim WA, Lee SG: Survival and growth of Cochlodinium polykrikoides red tide after addition of yellow loess. Journal of Environmental Biology2009, 30(6):929-932.[PHYTO-PAM]

            16.Marchetti A, Parker MS, Moccia LP, Lin EO, Arrieta AL, Ribalet F, Murphy MEP, Maldonado MT, Armbrust EV: Ferritin is used for iron storage in bloom-forming marine pennate diatoms. Nature2009, 457:467-470.[PHYTO-PAM]

            17.Morin S, Pesce S, Tlili A, Coste M, Montuelle B: Recovery potential of periphytic communities in a river impacted by a vineyard watershed Ecological Indicators2009, 10(2):419-426.[PHYTO-PAM]

            18.Nymark M, Valle KC, Brembu T, Hancke K, Winge P, Andresen K, Johnsen G, Bones AM: An Integrated Analysis of Molecular Acclimation to High Light in the Marine Diatom Phaeodactylum tricornutum. PLoS ONE2009, 4(11):e7743. doi:7710.1371/journal.pone.0007743.[PHYTO-PAM]

            19.Pesce S, Margoum C, Montuelle B: In situ relationships between spatio-temporal variations in diuron concentrations and phototrophic biofilm tolerance in a contaminated river. Water Research2009, 44(6):1941-1949.[PHYTO-PAM]

            20.Serra A, Corcoll N, Guasch H: Copper accumulation and toxicity in fluvial periphyton: The influence of exposure history Chemosphere2009, 74(5):633-641.[PHYTO-PAM]

            21.Serra A, Guasch H: Effects of chronic copper exposure on fluvial systems: Linking structural and physiological changes of fluvial biofilms with the in-stream copper retention. Science of The Total Environment2009, 407(19):5274-5282.[PHYTO-PAM]

            22.Serra A, Guasch H, Martí E, Geiszinger A: Measuring in-stream retention of copper by means of constant-rate additions Science of The Total Environment2009, 407(12):3847-3854.[PHYTO-PAM]

            23.Shi S, Tang D, Liu Y: Effects of an Algicidal Bacterium Pseudomonas mendocina on the Growth and Antioxidant System of Aphanizomenon flos-aquae Current Microbiology2009, 59(2):107-112.[PHYTO-PAM]

            24.Wu Z, Shi J, Li R: Comparative studies on photosynthesis and phosphate metabolism of Cylindrospermopsis raciborskii with Microcystis aeruginosa and Aphanizomenon flos-aquae Harmful Algae2009, 8(6):910-915.[PHYTO-PAM]

            25.Yang Z, Kong F, Yang Z, Zhang M, Yu Y, Qian S: Benefits and costs of the grazer-induced colony formation in Microcystis aeruginosa. Ann Limnol - Int J Lim2009, 45(3):203-208.[PHYTO-PAM]

            26.陳元, 趙洋甬, 潘雙葉, 徐運(yùn), 蔣蕾蕾: PHYTO-PAM對(duì)浮游植物中葉綠素的分類測(cè)定. 現(xiàn)代科學(xué)儀器2009(4):100-104.[PHYTO-PAM]

            27.朱曉敏, 黃清輝, 李建華: 咸水藻水華期溶解有機(jī)質(zhì)光譜特征變化的模擬. 中國(guó)環(huán)境科學(xué)2009, 29(1):68-72.[PHYTO-PAM]

            28.Brussaard CPD, Timmermans KR, Uitz J, Veldhuis MJW: Virioplankton dynamics and virally induced phytoplankton lysis versus microzooplankton grazing southeast of the Kerguelen (Southern Ocean) Deep Sea Research2008, 55(5-7):752-765.[PHYTO-PAM]

            29.Howeth JG, Leibold MA: Planktonic dispersal dampens temporal trophic cascades in pond metacommunities. Ecology Letters2008, 11(3):245-257.[PHYTO-PAM]

            30.Ingleton T, Kobayashi T, Sanderson B, Patra R, Macinnis-Ng CMO, Hindmarsh B, Bowling LC: Investigations of the temporal variation of cyanobacterial and other phytoplanktonic cells at the offtake of a large reservoir, and their survival following passage through it. Hydrobiologia2008, 603(1):221-240.[PHYTO-PAM]

            31.Schmitt-Jansen M, Altenburger R: Community-level microalgal toxicity assessment by multiwavelength-excitation PAM fluorometry Aquatic Toxicology2008, 86(1):49-58.[PHYTO-PAM]

            32.Timmermans KR, Veldhuis MJW, Laan P, Brussaard CPD: Probing natural iron fertilization near the Kerguelen (Southern Ocean) using natural phytoplankton assemblages and diatom cultures. DeepSeaResearch2008, 55(5-7):693-705.[PHYTO-PAM]

            33.Wang G, Chen K, Chen L, Hu C, Zhang D, Liu Y: The involvement of the antioxidant system in protection of desert cyanobacterium Nostoc sp. against UV-B radiation and the effects of exogenous antioxidants Ecotoxicology and Environmental Safety2008, 69(1):150-157.[PHYTO-PAM]

            34.Wu Z, Song L, Li R: Different tolerances and responses to low temperature and darkness between waterbloom forming cyanobacterium Microcystis and a green alga Scenedesmus Hydrobiologia2008, 596(1):47-55.[PHYTO-PAM]

            35.Wu Z-X, Song L-R: Physiological comparison between colonial and unicellular forms of Microcystis aeruginosa Kutz. (Cyanobacteria). Phycologia2008, 47(1):98-104.[PHYTO-PAM]

            36.Zhang M, Kong FX, Wu X, Xing P: Different photochemical responses of phytoplankters from the large shallow Taihu Lake of subtropical China in relation to light and mixing. In: Hydrobiologia. vol. 603; 2008: 267-278.

            37.胡智泉, 劉永定, 肖波: 微囊藻毒素對(duì)幾種淡水微藻的生長(zhǎng)和光合活性的影響. 生態(tài)環(huán)境2008, 17(3):885-890.[PHYTO-PAM]

            38.康麗娟, 潘曉潔, 常鋒毅, 李敦, 沈銀武, 劉永定: HCO3-堿度增加對(duì)銅綠微囊藻光合活性和超微結(jié)構(gòu)的影響. 武漢植物學(xué)研究2008, 26(1):70-75.[PHYTO-PAM]

            39.康麗娟, 潘曉潔, 常鋒毅, 李敦海, 沈銀武, 劉永定: 堿度增加對(duì)蛋白核小球藻光合活性與胞外多糖的影響. 湖泊科學(xué)2008, 20(2):251-256.[PHYTO-PAM]

            40.林燊, 彭欣, 吳忠興, 李仁輝: 我國(guó)水華藍(lán)藻的新類群——阿氏浮絲藻(Planktothrix agardhii)生理特性. 湖泊科學(xué)2008, 20(4):437-442.[PHYTO-PAM]

            41.蘇彥平, 李敦海, 王坎, 劉永定: 念珠藻葛仙米生理生化特性對(duì)不同低溫脅迫的響應(yīng). 武漢植物學(xué)研究2008, 26(3):310-314.[PHYTO-PAM]

            42.Alsterberg C, Sundbäck K, Larson F: Direct and indirect effects of an antifouling biocide on benthic microalgae and meiofauna Journal of Experimental Marine Biology and Ecology2007, 351(1-2):56-72.[PHYTO-PAM]

            43.Dimier C, Corato F, Saviello G, Brunet C: Photophysiological properties of the marine picoeukaryotePicochlorum RCC237 (Trebouxiophyceae, Chlorophyta). Journal of Phycology2007, 43(2):275-283.[PHYTO-PAM]

            44.Dimier C, Corato F, Tramontano F, Brunet C: Photoprotection and xanthophyll-cycle activity in three marine diatoms. Journal of Phycology2007, 43(5):937-947.[PHYTO-PAM]

            45.Domis LNDS, Mooij WM, Huisman J: Climate-induced shifts in an experimental phytoplankton community: a mechanistic approach. Hydrobiologia2007, 584:403-413.[PHYTO-PAM]

            46.Kim MK, Park JW, Park CS, Kim SJ, Jeune KH, Chang MU, Acreman J: Enhanced production of Scenedesmus spp. (green microalgae) using a new medium containing fermented swine wastewater. Bioresource Technology2007, 98(11):2220-2228.[PHYTO-PAM]

            47.Schmitt-Jansen M, Altenburger R: The use of pulse-amplitude modulated (PAM) fluorescence-based methods to evaluate effects of herbicides in microalgal systems of different complexity Toxicological and Environmental Chemistry2007, 89(4):665-681.[PHYTO-PAM, WATER-PAM, MICROSCOPY-PAM]

            48.Shen H, Song L-R: Comparative studies on physiological responses to phosphorus in two phenotypes of bloom-forming Microcystis. Hydrobiologia2007, 592:475-486.[PHYTO-PAM]

            49.Tang D, Shi S, Li D, Hu C, Liu Y: Physiological and biochemical responses of Scytonema javanicum (cyanobacterium) to salt stress Journal of Arid Environments2007, 71(3):312-320.[PHYTO-PAM]

            50.Wu Z-X, Gan N-Q, Huang Q, Song L-R: Response of Microcystis to copper stress - Do phenotypes of Microcystis make a difference in stress tolerance? Environmental Pollution2007, 147:324-330.[PHYTO-PAM]

            51.Xing W, Huang W-M, Li D-H, Liu Y-D: Effects of Iron on Growth, Pigment Content, Photosystem II Efficiency, and Siderophores Production of Microcystis aeruginosa and Microcystis wesenbergii Current Microbiology2007, 55:94-98.[PHYTO-PAM]

            52.Zhang M, Kong F, Xing P, Tan X: Effects of Interspecific Interactions between Microcystis aeruginosa and Chlorella pyrenoidosa on Their Growth and Physiology. International Review of Hydrobiology2007, 92(3):281-290.[PHYTO-PAM]

            53.陳麗芬, 鄭鋒: 葉綠素?zé)晒饧夹g(shù)快速測(cè)定水體藻類生物量的應(yīng)用. 城鎮(zhèn)供水2007(6):51-52.[PHYTO-PAM]

            54.康麗娟, 劉永梅, 李敦海, 劉永定: 不同鹽度下水華束絲藻對(duì)CO2濃度倍增的生理響應(yīng). 水生生物學(xué)報(bào)2007, 31(5):671-674.[PHYTO-PAM]

            55.劉永梅, 劉永定, 李敦海, 沈銀武: 氮磷對(duì)水華束絲藻生長(zhǎng)及生理特性的影響. 水生生物學(xué)報(bào)2007, 31(6):774-779.[PHYTO-PAM]

            56.吳曉東, 孔繁翔, 曹煥生, 張民, 劉桂民, 趙巧華: 越冬浮游植物光合作用活性的原位研究. 湖泊科學(xué)2007, 19(2):139-145.[PHYTO-PAM]

            57.張曼, 曾波: PhytoPAM浮游植物分析儀用于微藻光合作用研究中幾種參數(shù)設(shè)定的優(yōu)化. 植物生理學(xué)通訊2007, 43(1):148-152.[PHYTO-PAM]

            58.張曼, 曾波, 王明書, 吳國(guó)平, 任秋芳: 溫度升高對(duì)高光強(qiáng)環(huán)境下蛋白核小球藻(Chlolorella pyrenoidosa)光能利用和生長(zhǎng)的阻抑效應(yīng). 生態(tài)學(xué)報(bào)2007, 27(2):662-667.[PHYTO-PAM]

            59.Ban A, Aikawa S, Hattori H, Sasaki H, Sampei M, Kudoh S, Fukuchi M, Satoh K, Kashino Y: Comparative analysis of photosynthetic properties in ice algae and phytoplankton inhabiting Franklin Bay, the Canadian Arctic, with those in mesophilic diatoms during CASES 03-04. Polar Biosciences2006, 19:11-28.[PHYTO-PAM]

            60.Bontes BM, Pel R, Ibelings BW, Boschker HTS, Middelburg JJ, Donk EV: The effects of biomanipulation on the biogeochemistry, carbon isotopic composition and pelagic food web relations of a shallow lake. Biogeosciences2006, 3:69-83.[PHYTO-PAM]

            61.Hilt S, Ghobrial MGN, Gross EM: In situ allelopathic potential of Myriophyllum verticillatum (Haloragaceae) against selected phytoplankton species. Journal of Phycology2006, 42(6):1189-1198.[PHYTO-PAM]

            62.Liang Y, Beardall J, Heraud P: Changes in growth, chlorophyll fluorescence and fatty acid composition with culture age in batch cultures of Phaeodactylum tricornutum and Chaetoceros muelleri (Bacillariophyceae). Botanica Marina2006, 49(2):165-173.[PHYTO-PAM]

            63.Lürling M, Geest Gv, Scheffer M: Importance of Nutrient Competition and Allelopathic Effects in Suppression of the Green Alga Scenedesmus obliquus by the Macrophytes Chara, Elodea and Myriophyllum Hydrobiologia2006, 556(1):209-220.[PHYTO-PAM]

            64.Mulderij G, Smolders AJP, van Donk E: Allelopathic effect of the aquatic macrophyte, Stratiotes aloides, on natural phytoplankton. Freshwater Biology2006, 51(3):554-561.[PHYTO-PAM]

            65.Quigg A, Kevekordes K, Raven JA, Beardall J: Limitations on microalgal growth at very low photon fluence rates: the role of energy slippage Photosynthesis Research2006, 88(3):299-310.[PAM-2000, PHYTO-PAM]

            66.Roessink I, Belgers JDM, Crum SJH, van den Brink PJ, Brock TCM: Impact of triphenyltin acetate in microcosms simulating floodplain lakes. II. Comparison of species sensitivity distributions between laboratory and semi-field. Ecotoxicology2006, 15(5):411-424.[MINI-PAM, PHYTO-PAM]

            67.Bontes BM, Pel R, Ibelings BW, Boschker HTS, Middelburg JJ, Donk Ev: The effects of biomanipulation on the biogeochemistry, carbon isotopic composition and pelagic food web relations of a shallow turf lake. Biogeosciences Discussions2005, 2:997-1031.[PHYTO-PAM]

            68.Casotti R, Mazza S, Brunet C, Vantrepotte V, Ianora A, Miralto A: Growth inhibition and toxicity of the diatom aldehyde 2-trans, 4-trans-decadienal on Thalassiosira weissflogii (Bacillariophyceae). Journal of Phycology2005, 41(1):7-20.[PHYTO-PAM]

            69.Fietz S, Bleiß W, Hepperle D, Koppitz H, Krienitz L, Nicklisch A: First record of Nannochloropsis limnetica (Eustigmatophyceae) in the autotrophic picoplankton from lake Baikal. Journal of Phycology2005, 41(4):780-790.[PHYTO-PAM]

            70.Heraud P, Roberts S, Shelly K, Beardall J: Interations between UV-B exposure and phosphorus nutrition. II. Effects on rates of damage and repair. Journal of Phycology2005, 41(6):1212-1218.[PHYTO-PAM]

            71.Jakob T, Schreiber U, Kirchesch V, Langner U, Wilhelm C: Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwavelength-excitation PAM chlorophyll fluorometry: performance and methodological limits. Photosynthesis Research2005, 83:343–361.[PHYTO-PAM]

            72.Shelly K, Roberts S, Heraud P, Beardall J: Interactions between UV-B exposure and phosphorus nutrition. I. Effects on growth, phosphate uptake, and chlorophyll fluorescence. Journal of Phycology2005, 41(6):1204-1211.[PAM-2000, PHYTO-PAM]

            73.van derGrinten E, Janssen APHM, Mutsert Kd, Barranguet C, Admiraal W: Temperature- and light-dependent performance of the cyanobacterium Leptolyngbya foveolarum and the diatom Nitzschia perminuta in mixed biofilms. Hydrobiologia2005, 548(1):267-278.[PHYTO-PAM]

            74.Wang G, Chen L, Li G, Li D, Hu C, Chen H, Liu Y, Song L: Improving photosynthesis of microalgae by changing the ratio of light-harvesting pigments. Chinese Science Bulletin2005, 50(15):1622-1626.[PHYTO-PAM]

            75.Hu Z-Q, Liu Y-D, Li D-H: Physiological and biochemical analyses of microcystin-RR toxicity to the cyanobacterium Synechococcus elongatus. Environmental Toxicology2004, 19(6):571-577.[PHYTO-PAM]

            76.van der Grinten E, Janssen M, Simis SGH, Barranguet C, Admiraal W: Phosphate regime structures species composition in cultured phototrophic biofilms. Freshwater Biology2004, 49:369-381.[PHYTO-PAM]

            77.van der Grinten E, Simis S, Barranguet C, Admiraal W: Dominance of diatoms over cyanobacterial species in nitrogen-limited biofilms Archiv fuer Hydrobiologie 2004, 161(1):98-111.[PHYTO-PAM]

            78.Verspagen JMH, Snelder EOFM, Visser PM, Huisman J, Mur LR, Ibelings BW: Recruitment of benthic Microcystis (Cyanophyceae) to the water column: internal buoyancy changes or resuspension? Journal of Phycology2004, 40(2):260-270.[PHYTO-PAM]

            79.李闊宇, 宋立榮, **: 底泥中微囊藻復(fù)蘇和生長(zhǎng)特性的研究. 水生生物學(xué)報(bào)2004, 28(2):113-118.[PHYTO-PAM]

            80.Lurling M: Daphnia growth on microcystin-producing and microcystin-free Microcystis aeruginosa in different mixtures with the green alga Scenedesmus obliquus. Limnology and Oceanography2003, 48(6):2214-2220.[PHYTO-PAM]

            81.Lürling M, Verschoor AM: Fo-spectra of chlorophyll fluorescence for the determination of zooplankton grazing. Hydrobiologia2003, 491:145-157.[PHYTO-PAM]

            82.Mulderij G, Van Donk E, Roelofs2 GM: Differential sensitivity of green algae to allelopathic substances from Chara. Hydrobiologia2003, 491:261-271.[PHYTO-PAM]

            83.Verschoor AM, Takken J, Massieux B, Vijverberg J: The Limnotrons: a facility for experimental community and food web research. Hydrobiologia2003, 491:357-377.[PHYTO-PAM]

            84.Young EB, Beardall J: Photosynthetic function in Dunaliella tertiolecta (Chlorophyta) during a nitrogen starvation and recovery cycle. Journal of Phycology2003, 39(5):897-905.[PHYTO-PAM]

            85.Körner S, Nicklisch A: Allelopathic growth inhibition of selected phyplankton species by submerged macrophytes. Journal of Phycology2002, 38:862-871.[PHYTO-PAM]

            86.Schreiber U, Gademann R, Bird P, Ralph PJ, Larkum AWD, Kühl M: Apparent light requirement for activation of photosynthesis upon rehydration of desiccated beachrock microbial mats. Journal of Phycology2002, 38:125-134.[PHYTO-PAM]

            87.Nicklisch A, Köhler J: Estimatin of primary production with Phyto-PAM-fluorometry. Ann Report Inst Freshw Ecol Inland Fish Berlin2001, 13:47-60.[PHYTO-PAM]

            88.Varotto C, Pesaresi P, Maiwald D, Kurth J, Salamini F, Leister D: Identification of Photosynthetic mutants of Arabidopsis by automatic screening for altered effective quantum yield of photosystem 2. Photosynthetica2000, 38(4):497-504.[PAM-100, PHYTO-PAM]

            89.Schreiber U: Chlorophyll fluorescence: new instruments for special applications. In: Photosynthesis: Mechanisms and Effects. Edited by Garab G, vol. V. Dordrecht: Kluwer Academic Publishers; 1998.

            90.Kolbowski J, Schreiber U: Computer-controlled phytoplankton analyzer based on 4-wavelengths PAM chlorophyll fluorometer. In: Photosynthesis: from light to Biosphere. Edited by Mathis P, vol. V. Dordrecht: Kluwer Academic Publishers; 1995: 825-828.

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